Journal: Medicine
Article Title: Integrating network pharmacology and experimental verification to explore the mechanism of Tripterygium wilfordii in ankylosing spondylitis
doi: 10.1097/MD.0000000000036580
Figure Lengend Snippet: Expression of IL17 in 2 groups and determination of TP concentration. (A) The percentage of IL-17-positive CD4 + T cells in active AS patients tended to be elevated. (B) PBMC of patients with active AS were treated with different concentrations of TP (12.5–100 μg/mL). IC50 was 73.54 μg/mL for TP. (C) Decreased IL-17 concentration in the supernatant with 25-μg/mL TP; effect on cell proliferation was negligible. One-way ANOVA determined statistical significance. Compared with the blank control group, * : P < .05. PBMCs = Peripheral blood mononuclear cells.
Article Snippet: The cells were stained with anti-Human CD3 FITC (Product # 11-0037-42) and anti-Mouse CD4 FITC (Product # 11-0041-82) (eBioscience, USA).
Techniques: Expressing, Concentration Assay, Control